[pic] Faculty of Science Undergraduate Modular Scheme Session 2010/2011 Semester 2 Level 4 MODULE:LS1180: Genetics and Molecular Biology DATE:31 May 2011 TIME:09. 30 – 11. 30 Instructions to Candidates This paper contains THIRTY-EIGHT questions in FIVE sections: Sections A, B, C, D and E. Answer THIRTY-FOUR questions only Section A:THIS SECTION HAS BEEN WITHHELD BY THE FACULTY Answer ALL THIRTY questions on the MCQ sheet provided Section B:Answer ONE question Section C:Answer ONE question Section D:Answer ONE question Section E:Answer ONE questionSection A is worth 30 marks and Sections B to E are worth 15 marks each ANSWER SECTIONS B TO E IN SEPARATE ANSWER BOOKS CANDIDATES ARE PERMITTED TO BRING ONE APPROVED CALCULATOR INTO THIS EXAMINATION: from either Casio FX83 or Casio FX85 series (with any suffix), FX115MS, FX570ES or FX991ES Invigilators are under instruction to remove any other calculators Number of Pages: 1 – 9 SECTION A Each question has only ONE correct answer THIS SECTION HAS BEEN WITHHELD BY THE FACULTY Continued..
SECTION B Answer ONE question from this sectionFeature Article - Campbells Biology Review1.Draw an annotated diagram to illustrate the initiation of transcription in Eukaryotes. Label components in the diagram, as necessary, and briefly outline the process. (15 marks) 2.
Name five factors that influence the distribution of genes in a population to upset the Hardy Weinberg equilibrium, and describe each using an example (15 marks) SECTION C Answer ONE question from this section 3. The following is a pedigree of a family in which a rare form of colour blindness is found (filled-in symbols). Indicate as much as you can about the genotypes of all individuals in the pedigree. pic] (15 marks) 4.
A locus that affects susceptibility to a degenerative brain disease has two alleles, A and a. In a population, 16 people have genotype AA, 92 have genotype Aa and 12 have genotype aa. Is this population evolving? Explain. (15 marks) Continued.
.. SECTION D Answer ONE question from this section 5. Provide a brief description (using diagrams where appropriate) of the theory and methodology behind three of the techniques listed below that are commonly employed in molecular biology laboratories: Gel electrophoresis.
DNA sequencing. Polymerase chain reaction. cDNA synthesis. (15 marks) 36. What is heteroplasmy in terms of mitochondrial genetics and how does it arise (15 marks) SECTION E Answer ONE question from this section 37.
Summarise how natural selection works as a mechanism of evolution, illustrate your answer with two examples 15. marks) 38. Using diagrams explain what phylogenetic trees represent and describe monophyly, paraphyly and polyphyly (15 marks) END OF EXAMINATION PAPER ----------------------- 4 2 4 3 2 1 2 1 3 1 III II I
