Direction: Read the article below. Answer the questions beside the article by checking the number of the sentence where you can find the answer.
Then, write the specific answer on the space provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1234 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4567 ________________________________________ ______________________________________ c) How is chromatography done? 567 ____________________________________________________________ ___________________ 1)Chromatography is the name of the group of techniques that can be used to separate the components of mixtures. 2)Chromatography could not separate the components of mixtures.
3)Chromatography could not separate all mixtures but it can separate many different types of mixtures. 4)Solid, liquid and gaseous mixtures can be separated by chromatography. 5)Scientists have invented many different procedures of chromatography. 6)Some of these are gas chromatography, ion – exchange chromatography and adsorptive chromatography. ) What are the two parts of structures in chromatography? 78910 _______________________________________ d) The first thing that is common among all chromatographic procedures. 5678 _______________________________________ _________________________________________________ 7)Fortunately, all the different procedures of chromatography have two major things in common.
8)The first thing that is common among all chromatographic procedures is the structure of the laboratory set – up. 9)The structures will always have two parts. 10)These are the mobile phase and stationary phase. g) Why is solvent important in the mixtures? 12131415 ___________________________________________________________ ______________________ f) What happened to the mixture in the mobile phase? 11121314 ____________________________________________________________ ______________________ 11) The mobile phase is where the mixture to be separated is found. 12) The mixture may be separated at once or it may be dissolved first in a solvent.
13)The solvent, which is usually a liquid, helps the mixtures pass the stationary phase of the chromatographic laboratory set – up. 14)The stationary phase on the other hand, contains the substance that will be used to separate the components of the mixture. ------------------------------------------------ 15)The second thing is that is common in all chromatography procedures is the principle that governs the technique. 16)That is, the components of a mixture can be separated by using their distinct characteristics.
17)For example, a mixture is composed of two kinds of materials that differ in the size of their particles. 18)If the components of a mixture have particles that are different sizes, then the chromatographic procedures that can be used is the “molecular exclusion chromatography”. 19)The molecular exclusion chromatography is also known as gel filtration or gel permeation. 0) In this type of chromatography, the mobile phase will be the mixture that can be liquid or gaseous. 21)The mixtures pass the column that contains the stationary phase, which is porous gel. 22)The component of the mixture that contains smaller particles will pass through the porous gel faster than the component with larger particles.
------------------------------------------------- h) What is common in chromatography procedures? 13141516 _________________________________________ i) How will the components of the mixtures be separated? 14151617 _______________________________________ j) When can molecular exclusion chromatography used? 5161718 ____________________________________________________________ ______________________ k) What is the other name for molecular exclusion chromatorgraphy? 17181920 ________________________________________ l) What is the role of mobile phase in gel filtration? 19202122 ________________________________________ m) Where will the mixtures pass during gel filtration? 19202122 __________________________________________ n) How will the components of the mixture pass during gel filtration? 19202122 __________________________________________ 23)In the simplest types of chromatography, the interaction is usually adsorption. 4)Adsorption happens when particles stick to the surface of another material. 25)For example when the blackboard eraser is used, chalk dust is removed from the blackboard and is stuck to the surface of the eraser. 26)The chalk dust was adsorbed.
Materials adsorb differently. 27) Some adsorb strongly and these materials are describe as having great affinities. Some materials is adsorb weakly and they have lesser affinities. o) When will adsorption happen during chromatography? 22232425 ____________________________________________________________ ______________________ Prepared by: WENDY BALISACAN
